Laboratory for cyto- and molecular pathological analysis of systemic cancer

The diagnostic laboratory (systemic cancer progression laboratory, SCP-lab) of the Chair of Experimental Medicine and Therapy Research uses cytomorphological, cell biological and molecular pathological methods to investigate the systemic disease spread and molecular progression stage of cancer. The validated diagnostic procedures include the detection of circulating tumor cells in blood and disseminated tumor cells in bone marrow, lymph nodes, malignant effusions and CSF as well as their molecular-pathological characterization.

Analysis spectrum

  1. Immunocytological inspection of bone marrow, lymph nodes, blood, malignant effusions and CSF for disseminated and circulating tumor cells, including Her2 expression.
  2. Molecular pathological examination of disseminated and circulating tumor cells for genomic copy number alterations (CNA) or presence of a BRAFV600E/K Mutation

Note: Sample collection errors may result in false positive/negative results or make it impossible to analyse the sample. A negative result can never completely rule out the presence of systemic cancer, especially in the early stages of disease.

Information for phycisians

Type of sampleQuestionSample collectionAnticoagulant or fixativeAmountTransportationPossible investigation period after sample collection
Bone marrow• Hematogenous spread of epithelial tumors by detection of
    disseminated tumor cells
 • Detection of copy number alterations (CNA analysis)
Draw 5 ml of heparin (25,000 IU) into a 10 ml collection syringe before aspirating bone marrow into remaining syringe volume. Invert syringe carefully at least 10 times to ensure adequate heparin distribution.Heparinmax. 20 mlroom temperature, immediate delivery after collection (<60 min)same day
Lymph nodes• Lymphogenous spread of epithelial and melanocytic tumors by
   detection of disseminated tumor cells
• Detection of copy number alterations (CNA analysis)
• Detection of a BRAFV600E/K mutation
Storage and transport of lymph nodes in NaCl ---1-6 lymph nodesroom temperature, immediate delivery after excision (<60 min)same day
Blood • Detection of circulating tumor cells of epithelial tumors
   (CellSearch®), by request also with examination for HER2/neu
    expression
• Detection of copy number alterations (CNA analysis)
Please contact the laboratory for collection tubes: SCP-Lab@ukr.de
see below
Cell Save Preservative7,5 ml room temperature, delivery on the same or next day at the latest96 hours
CSF• Detection of circulating tumor cells of epithelial tumors, by request
also with examination for HER2/neu expression
• Detection of copy number alterations (CNA analysis)
 ---native≥ 3 mlroom temperature, immediate delivery after collection (<60 min)same day
Malignant effusions• Immunocytological quantification
• Detection of copy number alterations (CNA analysis)
• Detection of a BRAFV600E/K mutation 
 ---native≥ 10 mlroom temperature, immediate delivery after collection (<60 min)same day

General transport advice: Unfixed samples are potentially infectious. In order to rule out any risk to persons and the environment during transport, the sample must be packaged in a manner to avoid breakage.

Order forms

Please include the appropriate request form with each submission to the Chair of Experimental Medicine and Therapy Research.
Please request forms via: scp-lab@remove-this.ukr.de

Ordering of blood collection tubes for CellSearch®:

Please request via: scp-lab@remove-this.ukr.de

We are happy to receive your questions as well as other information relevant to the investigation. For inquiries, praise, criticism or suggestions, please contact us on:

phone: +49 941 944 6727
email: scp-lab@remove-this.ukr.de

Complaints and objections can be communicated to us by phone, in writing or by email:

Lehrstuhl für Experimentelle Medizin und Therapieverfahren
Prof. Christoph Klein

Universität Regensburg
Franz-Josef-Strauss Allee 11
93053 Regensburg

Tel.: +49 941 944 6727
E-Mail: scp-lab@remove-this.ukr.de

Upon receipt of a complaint, we will check whether the complaint relates to a service provided by us. If the unsatisfactory service falls within our area of responsibility, we will confirm this to you and endeavour to resolve the complaint as soon as possible. We ask for your understanding that a comprehensive clarification may take several weeks. Your complaint will always be dealt with impartially and non-discriminatorily.